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ELK Biotechnology
SKU:ELK9928
Rat DDAH2(Dimethylarginine Dimethylaminohydrolase 2) ELISA Kit
Rat DDAH2(Dimethylarginine Dimethylaminohydrolase 2) ELISA Kit
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Alternative Names: DDAH; G6A; NG30; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; Dimethylargininase-2; Protein G6a; S-phase protein
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DDAH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DDAH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DDAH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DDAH2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DDAH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DDAH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DDAH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DDAH2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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