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ELK Biotechnology
SKU:ELK9898
Sheep AMH(Anti-Mullerian Hormone) ELISA Kit
Sheep AMH(Anti-Mullerian Hormone) ELISA Kit
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$688.00 USD
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$688.00 USD
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Alternative Names: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
Assay Type: Sandwich
Sensitivity: 0.75 ng/mL
standard: 150 ng/mL
Detection range: 2.35-150 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep AMH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep AMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep AMH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep AMH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.75 ng/mL
standard: 150 ng/mL
Detection range: 2.35-150 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep AMH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep AMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep AMH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep AMH in the samples is then determined by comparing the OD of the samples to the standard curve.
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