1
/
of
1
ELK Biotechnology
SKU:ELK9880
Human DNA Methyltransferase 2 (DNMT2) ELISA Kit
Human DNA Methyltransferase 2 (DNMT2) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: TRDMT1; DNMT2; PUMET; RNMT1; MHSAIIP; AtDNMT2; DNA METHYLTRANSFERASE 2; DNA methyltransferase-2; T14C9.10; T14C9_10
Assay Type: Sandwich
Sensitivity: 26 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Enzyme & Kinase; Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNMT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNMT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNMT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNMT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 26 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Enzyme & Kinase; Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNMT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNMT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNMT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNMT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
