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ELK Biotechnology
SKU:ELK9817
EPA(Eicosapentaenoic Acid) ELISA Kit
EPA(Eicosapentaenoic Acid) ELISA Kit
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$558.00 USD
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Alternative Names: Icosapentaenoic Acid; Timnodonic Acid
Assay Type: Competitive Inhibition
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Biochemicals
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with EPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Biochemicals
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with EPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPA in the samples is then determined by comparing the OD of the samples to the standard curve.
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