1
/
of
1
ELK Biotechnology
SKU:ELK9816
Rat FHL1(Four And A Half LIM Domains Protein 1) ELISA Kit
Rat FHL1(Four And A Half LIM Domains Protein 1) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: FHL1B; KYO-T; SLIM1; XMPMA; FLH1A; LIM Protein SLIMMER; Skeletal muscle LIM-protein 1
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FHL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FHL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WUH4
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FHL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FHL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WUH4
Share
