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ELK Biotechnology
SKU:ELK9760
Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
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Alternative Names: ACA-IgM; Anti-Cardiolipin Antibody IgM
Assay Type: Sandwich
Sensitivity: 0.086 ng/mL
standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.086 ng/mL
standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
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