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ELK Biotechnology
SKU:ELK9457
Rat HK1(Hexokinase 1) ELISA Kit
Rat HK1(Hexokinase 1) ELISA Kit
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Alternative Names: HK1-Ta; HK1-Tb; HK1-Tc; HKI; HXK1; Brain form hexokinase
Assay Type: Sandwich
Sensitivity: 27.7 pg/mL
standard: 4000 pg/mL
Detection range: 62.5-4000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05708
Assay Type: Sandwich
Sensitivity: 27.7 pg/mL
standard: 4000 pg/mL
Detection range: 62.5-4000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05708
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