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ELK Biotechnology
SKU:ELK9402
Human AQPEP(Aminopeptidase Q) ELISA Kit
Human AQPEP(Aminopeptidase Q) ELISA Kit
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Alternative Names: AQPEP; AP-Q; Aminopeptidase Q; LVRN; CHL2 antigen; Laeverin
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Cell Biology; Developmental Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AQPEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AQPEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AQPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AQPEP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q6Q4G3
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Cell Biology; Developmental Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AQPEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AQPEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AQPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AQPEP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q6Q4G3
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