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ELK Biotechnology
SKU:ELK9342
Rat CERK(Ceramide Kinase) ELISA Kit
Rat CERK(Ceramide Kinase) ELISA Kit
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$558.00 USD
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Alternative Names: LK4; hCERK; Lipid kinase 4; Acylsphingosine kinase
Assay Type: Sandwich
Sensitivity: 1.16 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CERK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CERK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CERK in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 1.16 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CERK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CERK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CERK in the samples is then determined by comparing the OD of the samples to the standard curve.
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