Mouse SEMA4A(Semaphorin 4A) ELISA Kit

Alternative Names: CORD10; RP35; SEMAB; SEMB; Semaphorin-B; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4A
Assay Type: Sandwich
Sensitivity: 13.4 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SEMA4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SEMA4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SEMA4A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q62178