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ELK Biotechnology
SKU:ELK9207
Rat TNFSF4(Tumor Necrosis Factor Ligand Superfamily, Member 4) ELISA Kit
Rat TNFSF4(Tumor Necrosis Factor Ligand Superfamily, Member 4) ELISA Kit
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Alternative Names: CD252; TXGP1; OX40L; Gp34; OX40-L; Tax-Transcriptionally Activated Glycoprotein 1,34kD; Glycoprotein Gp34; OX40 ligand
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9Z2P3
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9Z2P3
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