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ELK Biotechnology
SKU:ELK9193
Human GSTs(Glutathione S Transferases) ELISA Kit
Human GSTs(Glutathione S Transferases) ELISA Kit
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Alternative Names: GSTs; GST-ST; GST ST
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTs. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTs, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTs in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60760
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTs. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTs, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTs in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60760
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