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ELK Biotechnology
SKU:ELK9081
Human GM(Aspergillus Galactomannan) ELISA Kit
Human GM(Aspergillus Galactomannan) ELISA Kit
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$558.00 USD
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Alternative Names: GM
Assay Type: Sandwich
Sensitivity: 9.34 pg/mL
standard: 1500 pg/mL
Detection range: 23.44-1500 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: bacterial infection
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 9.34 pg/mL
standard: 1500 pg/mL
Detection range: 23.44-1500 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: bacterial infection
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GM in the samples is then determined by comparing the OD of the samples to the standard curve.
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