1
/
of
1
ELK Biotechnology
SKU:ELK9079
Rat TEX101(testis expressed 101) ELISA Kit
Rat TEX101(testis expressed 101) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: Cell surface receptor NYD SP8; GTPR867; NYD SP8; PRO1884; SGRG; TES101RP; testis expressed 101; Testis expressed protein 101; TEX101
Assay Type: Sandwich
Sensitivity: 0.262 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TEX101. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TEX101. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TEX101, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TEX101 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q924B5
Assay Type: Sandwich
Sensitivity: 0.262 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TEX101. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TEX101. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TEX101, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TEX101 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q924B5
Share
