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ELK Biotechnology
SKU:ELK9034
Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
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Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
Assay Type: Competitive Inhibition
Sensitivity: 6.2 ng/mL
standard: 1000 ng/mL
Detection range: 15.6-1000 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIGF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q07326
Assay Type: Competitive Inhibition
Sensitivity: 6.2 ng/mL
standard: 1000 ng/mL
Detection range: 15.6-1000 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIGF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q07326
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