1
/
of
1
ELK Biotechnology
SKU:ELK8754
Plant GA(Gibberellic Acid) ELISA Kit
Plant GA(Gibberellic Acid) ELISA Kit
Regular price
$476.00 USD
Regular price
Sale price
$476.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: GA3; Gibberellin A3; Gibberellins
Assay Type: Competitive Inhibition
Sensitivity: 52 ng/mL
standard: 10000 ng/mL
Detection range: 156.25-10000 ng/mL
Sample type: plant tissues
Assay length: 2.5h
Research Area: Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant GA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant GA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 52 ng/mL
standard: 10000 ng/mL
Detection range: 156.25-10000 ng/mL
Sample type: plant tissues
Assay length: 2.5h
Research Area: Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant GA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant GA in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
