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ELK Biotechnology
SKU:ELK8628
Plant CYT(Cytokinin) ELISA Kit
Plant CYT(Cytokinin) ELISA Kit
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$558.00 USD
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Alternative Names: CYT
Assay Type: Competitive Inhibition
Sensitivity: 0.064 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: plant tissues
Assay length: 2.5h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant CYT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant CYT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant CYT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.064 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: plant tissues
Assay length: 2.5h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant CYT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant CYT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant CYT in the samples is then determined by comparing the OD of the samples to the standard curve.
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