1
/
of
1
ELK Biotechnology
SKU:ELK8552
Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit
Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F
Assay Type: Sandwich
Sensitivity: 26.14 pg/mL
standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Breast Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P63074
Assay Type: Sandwich
Sensitivity: 26.14 pg/mL
standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Breast Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P63074
Share
