Skip to product information
1 of 1

ELK Biotechnology

SKU:ELK8432

Human GA(Glycated Albumin) ELISA Kit

Human GA(Glycated Albumin) ELISA Kit

Regular price $558.00 USD
Regular price Sale price $558.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Alternative Names: Glycated Albumin,GA
Assay Type: Competitive Inhibition
Sensitivity: 5.03 pmol/mL
standard: 1000 pmol/mL
Detection range: 15.63-1000 pmol/mL
Sample type: serum, plasma
Assay length: 2.5h
Research Area: diabetic nephropathy
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GA in the samples is then determined by comparing the OD of the samples to the standard curve.
View full details