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ELK Biotechnology
SKU:ELK8414
Mouse dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit
Mouse dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit
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Alternative Names: dsDNA Ab IgG
Assay Type: Sandwich
Sensitivity: 0.092 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.092 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
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