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ELK Biotechnology
SKU:ELK8403
Rat STOML2 ELISA Kit
Rat STOML2 ELISA Kit
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Alternative Names: SLP-2; Paratarg-7; EPB72-like protein 2; Paraprotein target 7
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.156-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: tumor marker
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STOML2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STOML2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STOML2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat STOML2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q4FZT0
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.156-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: tumor marker
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STOML2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STOML2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STOML2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat STOML2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q4FZT0
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