1
/
of
1
ELK Biotechnology
SKU:ELK8284
SB(Stercobilin) ELISA Kit
SB(Stercobilin) ELISA Kit
Regular price
$476.00 USD
Regular price
Sale price
$476.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: SB
Assay Type: Competitive Inhibition
Sensitivity: 0.58 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma, stool and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.58 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma, stool and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SB in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
