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ELK Biotechnology
SKU:ELK8259
Pig CLPS(Colipase, Pancreatic) ELISA Kit
Pig CLPS(Colipase, Pancreatic) ELISA Kit
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$688.00 USD
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Alternative Names: clpS
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CLPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CLPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CLPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CLPS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02703
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CLPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CLPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CLPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CLPS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02703
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