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ELK Biotechnology
SKU:ELK8090
Pig Plg(Plasminogen) ELISA Kit
Pig Plg(Plasminogen) ELISA Kit
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Alternative Names: PL; Plasmin; Activation peptide; Angiostatin
Assay Type: Sandwich
Sensitivity: 1.48 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Plg in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06867
Assay Type: Sandwich
Sensitivity: 1.48 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Plg in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06867
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