1
/
of
1
ELK Biotechnology
SKU:ELK7983
Pig FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit
Pig FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit
Regular price
$688.00 USD
Regular price
Sale price
$688.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein
Assay Type: Sandwich
Sensitivity: 0.26 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O02772
Assay Type: Sandwich
Sensitivity: 0.26 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O02772
Share
