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ELK Biotechnology
SKU:ELK7933
Mouse CD5L(CD5 Antigen Like Protein) ELISA Kit
Mouse CD5L(CD5 Antigen Like Protein) ELISA Kit
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Alternative Names: API6; CT-2; SP-ALPHA; Spalpha; Apoptosis Inhibitor 6; Scavenger Receptor Cysteine Rich Family; IgM-associated peptide
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD5L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD5L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD5L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD5L in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9QWK4
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD5L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD5L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD5L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD5L in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9QWK4
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