1
/
of
1
ELK Biotechnology
SKU:ELK7921
HVA(Homovanillic Acid) ELISA Kit
HVA(Homovanillic Acid) ELISA Kit
Regular price
$476.00 USD
Regular price
Sale price
$476.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid
Assay Type: Competitive Inhibition
Sensitivity: 0.96 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Neuroscience
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.96 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Neuroscience
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
