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ELK Biotechnology
SKU:ELK7759
Mouse Igk(Immunoglobulin Kappa) ELISA Kit
Mouse Igk(Immunoglobulin Kappa) ELISA Kit
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Alternative Names: IGKC; HCAK1; Km; Immunoglobulin Kappa Constant; IgA Kappa
Assay Type: Sandwich
Sensitivity: 35 ng/mL
standard: 5000 ng/mL
Detection range: 78.13-5000 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Igk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Igk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Igk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Igk in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01837
Assay Type: Sandwich
Sensitivity: 35 ng/mL
standard: 5000 ng/mL
Detection range: 78.13-5000 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Igk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Igk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Igk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Igk in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01837
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