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ELK Biotechnology
SKU:ELK7727
Human CACNg6(Calcium Channel, Voltage Dependent, Gamma Subunit 6) ELISA Kit
Human CACNg6(Calcium Channel, Voltage Dependent, Gamma Subunit 6) ELISA Kit
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Alternative Names: Neuronal voltage-gated calcium channel gamma-6 subunit
Assay Type: Sandwich
Sensitivity: 0.111 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CACNg6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CACNg6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CACNg6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CACNg6 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9BXT2
Assay Type: Sandwich
Sensitivity: 0.111 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CACNg6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CACNg6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CACNg6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CACNg6 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9BXT2
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