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ELK Biotechnology
SKU:ELK7591
Human TMEM173(Transmembrane Protein 173) ELISA Kit
Human TMEM173(Transmembrane Protein 173) ELISA Kit
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Alternative Names: NET23; ERIS; MPYS; MITA; STING; Endoplasmic Reticulum Interferon Stimulator; Stimulator Of Interferon Genes Protein; Mediator of IRF3 activation
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM173. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM173. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM173, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM173 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q86WV6
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM173. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM173. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM173, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM173 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q86WV6
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