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ELK Biotechnology
SKU:ELK7410
Mouse TPM3(Tropomyosin 3) ELISA Kit
Mouse TPM3(Tropomyosin 3) ELISA Kit
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Alternative Names: NEM1; TRK; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; Hscp30; Gamma-tropomyosin
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPM3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPM3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21107
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPM3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPM3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21107
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