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ELK Biotechnology
SKU:ELK7266
Rat XBP1(X-Box Binding Protein 1) ELISA Kit
Rat XBP1(X-Box Binding Protein 1) ELISA Kit
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Alternative Names: TREB5; XBP2; Tax-responsive element-binding protein 5
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat XBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat XBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat XBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat XBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9R1S4
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat XBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat XBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat XBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat XBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9R1S4
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