Rat PDGFD(Platelet Derived Growth Factor D) ELISA Kit

Alternative Names: PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDGFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PDGFD in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9EQT1