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ELK Biotechnology
SKU:ELK7109
Mouse SPTLC2(Serine Palmitoyltransferase, Long Chain Base Subunit 2) ELISA Kit
Mouse SPTLC2(Serine Palmitoyltransferase, Long Chain Base Subunit 2) ELISA Kit
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Alternative Names: LCB2; SPT2; Long chain base biosynthesis protein 2; Serine-palmitoyl-CoA transferase 2
Assay Type: Sandwich
Sensitivity: 0.052 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SPTLC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SPTLC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SPTLC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SPTLC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P97363
Assay Type: Sandwich
Sensitivity: 0.052 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SPTLC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SPTLC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SPTLC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SPTLC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P97363
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