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ELK Biotechnology
SKU:ELK6986
Mouse COL6(Collagen Type VI) ELISA Kit
Mouse COL6(Collagen Type VI) ELISA Kit
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$558.00 USD
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Alternative Names: collagen 6; OPLL ; PP3610
Assay Type: Sandwich
Sensitivity: 1.25 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 1.25 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
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