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ELK Biotechnology
SKU:ELK6973
Mouse HSD17b10(17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit
Mouse HSD17b10(17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit
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Alternative Names: 17b-HSD10; ABAD; ERAB; HADH2; HCD2; MHBD; SCHAD; SDR5C1, MRPP2; CAMR; 3-Hydroxyacyl-CoA Dehydrogenase Type-2; AB-Binding Alcohol Dehydrogenase
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSD17b10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSD17b10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSD17b10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSD17b10 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O08756
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSD17b10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSD17b10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSD17b10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSD17b10 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O08756
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