1
/
of
1
ELK Biotechnology
SKU:ELK6954
Mouse PHF8(PHD Finger Protein 8) ELISA Kit
Mouse PHF8(PHD Finger Protein 8) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: ZNF422; MRXSSD; JHDM1F; Jumonji C Domain-Containing Histone Demethylase 1F; Histone lysine demethylase PHF8
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PHF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PHF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PHF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PHF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q80TJ7
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PHF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PHF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PHF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PHF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q80TJ7
Share
