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ELK Biotechnology
SKU:ELK6807
Rat SCNN1a(Amiloride Sensitive Sodium Channel Subunit Alpha) ELISA Kit
Rat SCNN1a(Amiloride Sensitive Sodium Channel Subunit Alpha) ELISA Kit
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Alternative Names: ENaCa; ENaCalpha; SCNEA; SCNN1; Sodium Channel,Nonvoltage-Gated 1,Alpha; Epithelial Na(+) channel subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha
Assay Type: Sandwich
Sensitivity: 0.062 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SCNN1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCNN1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SCNN1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCNN1a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P37089
Assay Type: Sandwich
Sensitivity: 0.062 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SCNN1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCNN1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SCNN1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCNN1a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P37089
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