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ELK Biotechnology
SKU:ELK6806
Mouse MTPN(Myotrophin) ELISA Kit
Mouse MTPN(Myotrophin) ELISA Kit
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Alternative Names: GCDP; V-1; Granule Cell Differentiation Protein
Assay Type: Sandwich
Sensitivity: 2.57 pg/mL
standard: 400 pg/mL
Detection range: 6.25-400 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MTPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MTPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MTPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MTPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P62774
Assay Type: Sandwich
Sensitivity: 2.57 pg/mL
standard: 400 pg/mL
Detection range: 6.25-400 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MTPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MTPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MTPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MTPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P62774
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