Rat THRa(Thyroid Hormone Receptor Alpha) ELISA Kit

Alternative Names: THR-A; NR1A1; AR7; EAR7; ERB-T1; ERBA1; NR1-A1; THRA1; THRA2; c-ERBA1; Erythroblastic Leukemia Viral Oncogene Homolog; Nuclear Receptor Subfamily 1,Group A,Member 1
Assay Type: Sandwich
Sensitivity: 0.272 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat THRa in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P63059