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ELK Biotechnology
SKU:ELK6559
Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) ELISA Kit
Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) ELISA Kit
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Alternative Names: MyHC-peri; MyHC-pn; MyHC-perinatal; Myosin heavy chain, skeletal muscle, perinatal
Assay Type: Sandwich
Sensitivity: 25.5 pg/mL
standard: 4000 pg/mL
Detection range: 62.5-4000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04462
Assay Type: Sandwich
Sensitivity: 25.5 pg/mL
standard: 4000 pg/mL
Detection range: 62.5-4000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04462
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