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ELK Biotechnology
SKU:ELK6502
Human MARCKSL1(MARCKS Related Protein) ELISA Kit
Human MARCKSL1(MARCKS Related Protein) ELISA Kit
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Alternative Names: MLP1; MRP; MLP; F52; MACMARCKS; MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MARCKSL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MARCKSL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MARCKSL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MARCKSL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P49006
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MARCKSL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MARCKSL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MARCKSL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MARCKSL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P49006
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