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ELK Biotechnology
SKU:ELK6457
Mouse THBS3(Thrombospondin 3) ELISA Kit
Mouse THBS3(Thrombospondin 3) ELISA Kit
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Alternative Names: TSP3
Assay Type: Sandwich
Sensitivity: 25 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Plasma.
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse THBS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse THBS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse THBS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse THBS3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q05895
Assay Type: Sandwich
Sensitivity: 25 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Plasma.
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse THBS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse THBS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse THBS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse THBS3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q05895
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