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ELK Biotechnology
SKU:ELK6437
Rat PFKM(Phosphofructokinase, Muscle) ELISA Kit
Rat PFKM(Phosphofructokinase, Muscle) ELISA Kit
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Alternative Names: PFK1; PFKX; Phosphofructokinase 1; Phosphofructo-1-kinase isozyme A; 6-Phosphofructokinase Polypeptide X; Fructose-6-Phosphate 1-Phosphotransferase
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PFKM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PFKM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PFKM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PFKM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P47858
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PFKM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PFKM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PFKM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PFKM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P47858
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