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ELK Biotechnology
SKU:ELK6232
Mouse PSPN(Persephin) ELISA Kit
Mouse PSPN(Persephin) ELISA Kit
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Alternative Names: PSP
Assay Type: Sandwich
Sensitivity: 7.7 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O70300
Assay Type: Sandwich
Sensitivity: 7.7 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O70300
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