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ELK Biotechnology
SKU:ELK6229
Rat AhR(Aryl Hydrocarbon Receptor) ELISA Kit
Rat AhR(Aryl Hydrocarbon Receptor) ELISA Kit
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Alternative Names: BHLHE76; Class E basic helix-loop-helix protein 76
Assay Type: Sandwich
Sensitivity: 0.105 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AhR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AhR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AhR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AhR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P41738
Assay Type: Sandwich
Sensitivity: 0.105 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AhR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AhR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AhR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AhR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P41738
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