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ELK Biotechnology
SKU:ELK6201
Human AGGF1(Angiogenic Factor With G Patch And FHA Domains 1) ELISA Kit
Human AGGF1(Angiogenic Factor With G Patch And FHA Domains 1) ELISA Kit
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Alternative Names: GPATC7; GPATCH7; VG5Q; Angiogenic factor VG5Q; G patch domain-containing protein 7; Vasculogenesis gene on 5q protein
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8N302
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8N302
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