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ELK Biotechnology
SKU:ELK5840
Cattle IGF2(Insulin Like Growth Factor 2) ELISA Kit
Cattle IGF2(Insulin Like Growth Factor 2) ELISA Kit
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Alternative Names: INSIGF; IGF-II; Somatomedin A; Preptin; Insulin-like growth factor II Ala-25 Del
Assay Type: Sandwich
Sensitivity: 0.287 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P07456
Assay Type: Sandwich
Sensitivity: 0.287 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P07456
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