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ELK Biotechnology
SKU:ELK5795
Cattle TLR4(Toll Like Receptor 4) ELISA Kit
Cattle TLR4(Toll Like Receptor 4) ELISA Kit
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Alternative Names: CD284; TOLL; HToll
Assay Type: Sandwich
Sensitivity: 0.243 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TLR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TLR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TLR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TLR4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9GL65
Assay Type: Sandwich
Sensitivity: 0.243 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TLR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TLR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TLR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TLR4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9GL65
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