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ELK Biotechnology
SKU:ELK5698
Rat Bax(Bcl2 Associated X Protein) ELISA Kit
Rat Bax(Bcl2 Associated X Protein) ELISA Kit
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Alternative Names: BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4
Assay Type: Sandwich
Sensitivity: 4.7 pg/mL
standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bax. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bax. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bax, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bax in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q63690
Assay Type: Sandwich
Sensitivity: 4.7 pg/mL
standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bax. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bax. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bax, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bax in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q63690
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